FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_1_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_1_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30221343
Sequences flagged as poor quality0
Sequence length10-38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN4355531.441209942258357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2487390.8230574001956167No Hit
GGCACCCGAGAATTCCA679040.22468888957052638Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT493540.1633084274249493No Hit
GCACCCGAGAATTCCA400480.13251561983860213Illumina Small RNA Adapter 2 (100% over 16bp)
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA358730.11870087970610704No Hit
CGAGAATTCCA319670.10577623899771761Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA307000.10158383762098196Illumina Small RNA Adapter 2 (100% over 12bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)