FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_40_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_40_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11331720
Sequences flagged as poor quality0
Sequence length10-38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1304981.1516168772260522No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN858910.7579696639168634No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA499550.44084216694376493No Hit
GGCACCCGAGAATTCCA483470.4266519116250666Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT391190.34521678968417857No Hit
GCACCCGAGAATTCCA303870.268158761423685Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA262380.23154472577861085Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA234660.20708241996801896Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA202140.1783842170473679Illumina Small RNA Adapter 2 (100% over 14bp)
CGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT142820.12603558859555303No Hit
CCCGAGAATTCCA128180.11311610240987247Illumina Small RNA Adapter 2 (100% over 13bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)