FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_40_R2_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_40_R2_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12343233
Sequences flagged as poor quality0
Sequence length10-30
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN3503612.8384864808109835No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC2841032.3016903269994176No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTT286880.23241884844918667No Hit
CGCGGGCCCAGAAGCGCAGCGACAGCCTC215920.17492985832804098No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN159890.1295365646909525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)