FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_50_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_50_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14164231
Sequences flagged as poor quality0
Sequence length10-38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1636561.1554174737760208No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN1070170.7555440178856163No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA623840.4404333705091367No Hit
GGCACCCGAGAATTCCA604430.4267298379982648Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT488380.34479810446468995No Hit
GCACCCGAGAATTCCA378790.2674271550640483Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA328810.2321410883513549Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA292300.2063648919591893Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA253470.17895076690008796Illumina Small RNA Adapter 2 (100% over 14bp)
CGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT179630.1268194510524433No Hit
CCCGAGAATTCCA160630.11340538007322812Illumina Small RNA Adapter 2 (100% over 13bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)