FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_60_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_60_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16997812
Sequences flagged as poor quality0
Sequence length10-38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1964151.155531076587975No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN1283360.7550148219076667No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA747100.4395271579659782No Hit
GGCACCCGAGAATTCCA725910.4270608475961495Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT588050.3459562913156117No Hit
GCACCCGAGAATTCCA455310.26786388742268713Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA394790.23225930490347818Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA350340.2061088803664848Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA304340.17904657375902264Illumina Small RNA Adapter 2 (100% over 14bp)
CGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT213900.12583972572469915No Hit
CCCGAGAATTCCA192300.11313220783945604Illumina Small RNA Adapter 2 (100% over 13bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)