FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_70_R2_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_70_R2_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21602590
Sequences flagged as poor quality0
Sequence length10-30
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN6127392.836414522517902No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC4977722.3042237065092657No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTT502910.23280078916463257No Hit
CGCGGGCCCAGAAGCGCAGCGACAGCCTC378350.17514103632944011No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN281190.13016494781412785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)