FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_90_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_90_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25498419
Sequences flagged as poor quality0
Sequence length10-38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2945481.1551618161110302No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN1928990.7565135705080381No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA1117670.4383291371908195No Hit
GGCACCCGAGAATTCCA1092470.42844617150577063Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT881280.3456214285285688No Hit
GCACCCGAGAATTCCA681720.2673577526512526Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA593850.23289679254231407Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA524550.2057186369084295Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA457170.17929346913626293Illumina Small RNA Adapter 2 (100% over 14bp)
CGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT319990.1254940551412227No Hit
CCCGAGAATTCCA289350.11347762384797269Illumina Small RNA Adapter 2 (100% over 13bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)