FastQCFastQC Report
Mon 15 Jun 2020
H9_treated_PRO-seq_1_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_treated_PRO-seq_1_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29119715
Sequences flagged as poor quality0
Sequence length10-38
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN2568000.8818767628735378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2091150.7181217261226629No Hit
GGCACCCGAGAATTCCA1053170.3616690616649236Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT1009880.34680284473938017No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA713880.24515349823993815No Hit
GCACCCGAGAATTCCA662850.22762928826741607Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA527300.18108006894985065Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA465000.15968562879135323Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA426940.14661544592726955Illumina Small RNA Adapter 2 (100% over 14bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)