FastQCFastQC Report
Mon 15 Jun 2020
H9_treated_PRO-seq_1_R2_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_treated_PRO-seq_1_R2_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31193330
Sequences flagged as poor quality0
Sequence length10-30
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN9181202.943321537008072No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC5478361.756260072265449No Hit
CGCGGGCCCAGAAGCGCAGCGACAGCCTC534640.17139561566527203No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTT446470.14312995759029254No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN388350.12449776923464087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)