FastQCFastQC Report
Mon 15 Jun 2020
H9_treated_PRO-seq_2_R2_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_treated_PRO-seq_2_R2_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30761688
Sequences flagged as poor quality0
Sequence length10-30
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN8242132.679349065629949No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC6713072.1822827147847024No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTT578040.1879090640279558No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN400060.13005138079548823No Hit
CGCGGGCCCAGAAGCGCAGCGACAGCCTC371420.12074109847287964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)