FastQCFastQC Report
Mon 15 Jun 2020
H9_treated_PRO-seq_3_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_treated_PRO-seq_3_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32320459
Sequences flagged as poor quality0
Sequence length10-38
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN3322641.0280299546488494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1989980.6157028896155219No Hit
GGCACCCGAGAATTCCA706420.21856744051809412Illumina Small RNA Adapter 2 (100% over 17bp)
GCACCCGAGAATTCCA492880.1524978342665245Illumina Small RNA Adapter 2 (100% over 16bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT371650.11498908477753983No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA336570.10413527852435511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)