FastQCFastQC Report
Mon 15 Jun 2020
HEK_PRO-seq_R2_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHEK_PRO-seq_R2_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20545848
Sequences flagged as poor quality0
Sequence length10-30
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC5043312.4546613992277178No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN2839331.3819483138393702No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTT746670.36341649174081303No Hit
CGCGGGCCCAGAAGCGCAGCGACAGCCTC407130.19815682467815396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)