FastQCFastQC Report
Thu 28 May 2020
K562_RNA-seq_40_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameK562_RNA-seq_40_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68949770
Sequences flagged as poor quality0
Sequence length2-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT823170.11938691021014285No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG779840.11310262528794511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)