Streaming uncompressed output to STDOUT... Read1 before filtering: total reads: 1000000 total bases: 37678766 Q20 bases: 34709953(92.1207%) Q30 bases: 34018346(90.2852%) Read1 after filtering: total reads: 439519 total bases: 16134044 Q20 bases: 15454671(95.7892%) Q30 bases: 15178418(94.077%) Filtering result: reads passed filter: 439519 reads failed due to low quality: 66799 reads failed due to too many N: 105 reads failed due to too short: 493577 reads with adapter trimmed: 540203 bases trimmed due to adapters: 14696777 Duplication rate (may be overestimated since this is SE data): 28.6979% JSON report: /sfs/lustre/allocations/shefflab/processed/peppro_tutorial/pe/07-31-19/results_pipeline/tutorial/fastqc/tutorial_R2_rmAdapter.json HTML report: /sfs/lustre/allocations/shefflab/processed/peppro_tutorial/pe/07-31-19/results_pipeline/tutorial/fastqc/tutorial_R2_rmAdapter.html fastp --overrepresentation_analysis --thread 1 --in1 /sfs/lustre/allocations/shefflab/processed/peppro_tutorial/pe/07-31-19/results_pipeline/tutorial/fastq/tutorial_R2.fastq --adapter_sequence GATCGTCGGACTGTAGAACTCTGAAC --length_required 26 --html /sfs/lustre/allocations/shefflab/processed/peppro_tutorial/pe/07-31-19/results_pipeline/tutorial/fastqc/tutorial_R2_rmAdapter.html --json /sfs/lustre/allocations/shefflab/processed/peppro_tutorial/pe/07-31-19/results_pipeline/tutorial/fastqc/tutorial_R2_rmAdapter.json --report_title tutorial --stdout fastp v0.19.4, time used: 7 seconds