{
"summary": {
"before_filtering": {
"total_reads":1000000,
"total_bases":37678766,
"q20_bases":34709953,
"q30_bases":34018346,
"q20_rate":0.921207,
"q30_rate":0.902852,
"read1_mean_length":37,
"gc_content":0.518639
},
"after_filtering": {
"total_reads":439519,
"total_bases":16134044,
"q20_bases":15454671,
"q30_bases":15178418,
"q20_rate":0.957892,
"q30_rate":0.94077,
"read1_mean_length":36,
"gc_content":0.526951
}
},
"filtering_result": {
"passed_filter_reads": 439519,
"low_quality_reads": 66799,
"too_many_N_reads": 105,
"too_short_reads": 493577,
"too_long_reads": 0
},
"duplication": {
"rate": 0.286979,
"histogram": [437812,16546,8852,5892,3938,2938,2123,1537,1168,918,718,552,445,368,315,274,220,184,152,137,120,87,94,90,79,61,49,39,34,34,295],
"mean_gc": [0.449334,0.458295,0.463191,0.461576,0.462608,0.464017,0.468745,0.467297,0.462957,0.463121,0.46369,0.469295,0.4685,0.457651,0.458724,0.475097,0.462531,0.457651,0.462126,0.454845,0.463007,0.469732,0.473467,0.462789,0.461703,0.471681,0.455542,0.464957,0.471742,0.467013,0.471545]
},
"adapter_cutting": {
"adapter_trimmed_reads": 540203,
"adapter_trimmed_bases": 14696777,
"read1_adapter_sequence": "GATCGTCGGACTGTAGAACTCTGAAC",
"read1_adapter_counts": {"GATCGTCGG":14299, "GATCGTCGGAC":6547, "GATCGTCGGACT":5538, "GATCGTCGGACTG":5881, "GATCGTCGGACTGT":17243, "GATCGTCGGACTGTAGAACTCTGAACGTG":7306, "GATCGTCGGACTGTAGAACTCTGAACGTGT":404556, "others":78833}
},
"read1_before_filtering": {
"total_reads": 1000000,
"total_bases": 37678766,
"q20_bases": 34709953,
"q30_bases": 34018346,
"total_cycles": 38,
"quality_curves": {
"A":[30.1023,29.9909,30.3424,30.3731,30.3951,33.7563,33.8171,33.7964,33.6769,34.2461,32.0416,32.2345,33.3671,31.4002,32.1319,33.9531,33.8651,34.3952,32.5273,31.4524,33.4457,31.626,34.0713,33.6001,34.1499,34.3216,32.0106,31.1379,31.4294,31.0495,33.149,34.0174,34.3201,32.2479,33.1344,30.9166,32.6897,34.06],
"T":[30.9601,30.7757,30.8734,30.8686,30.8783,34.3984,34.4052,34.4866,34.7508,34.6364,34.4516,34.4478,34.6493,34.0627,34.3648,34.7592,34.7462,34.717,34.5812,34.1128,34.6857,34.1296,34.6354,34.7421,34.6366,34.6568,34.4945,34.254,34.1913,33.9201,34.6827,34.6854,34.6539,34.5645,34.5903,33.921,34.6256,34.0429],
"C":[31.0626,30.9065,30.9966,31.0003,31.0258,34.5989,34.5798,34.6138,34.8344,34.713,34.6296,34.3718,34.7863,34.5195,34.3702,34.7335,34.8514,34.793,34.5575,34.5447,34.835,34.5791,34.7124,34.8038,34.7131,34.6841,34.6826,34.4275,34.1128,34.3422,34.7444,34.718,34.6531,34.6465,34.7181,34.5319,34.7889,34.3973],
"G":[30.5229,30.3815,30.5511,30.5992,30.5904,34.0087,34.0169,34.0197,33.9322,33.8345,34.3164,34.2746,33.6745,34.1624,34.1708,33.8907,34.0021,33.9368,34.3762,34.1284,33.6978,34.1745,33.7549,33.9866,33.8954,33.9569,34.3227,34.1765,34.0206,33.835,33.573,33.7451,33.8577,34.2241,33.6588,33.9831,33.671,34.0425],
"mean":[30.1568,30.0866,30.2723,30.2905,30.2976,33.7199,33.7054,33.7193,33.6292,33.7876,33.6127,33.5872,33.4341,33.3069,33.5387,33.6331,33.6975,33.9122,33.7498,33.3164,33.4659,33.324,33.6714,33.629,33.7399,33.8571,33.7149,33.356,33.2514,33.0513,33.3197,33.645,33.8238,33.7259,33.3852,33.6214,33.8441,34.06]
},
"content_curves": {
"A":[0.314654,0.237083,0.219982,0.220298,0.215974,0.21456,0.233767,0.22907,0.137049,0.552003,0.141955,0.130698,0.12543,0.128143,0.132591,0.129813,0.141034,0.548306,0.138182,0.136471,0.133899,0.150379,0.552425,0.159106,0.562527,0.555956,0.14243,0.132202,0.133747,0.14431,0.14802,0.555425,0.5493,0.13679,0.12267,0.120523,0.10077,0],
"T":[0.194407,0.21312,0.219779,0.222531,0.216976,0.212131,0.226769,0.238401,0.123604,0.128531,0.541415,0.130619,0.137744,0.545369,0.124522,0.118915,0.120976,0.120502,0.130283,0.54418,0.13973,0.534456,0.124401,0.118367,0.121606,0.121829,0.132758,0.539629,0.136758,0.534435,0.132143,0.119065,0.121794,0.131477,0.14243,0.561881,0.156361,0.675335],
"C":[0.185744,0.249803,0.268543,0.269994,0.273495,0.28595,0.241631,0.219478,0.135682,0.129244,0.140163,0.554351,0.137444,0.137638,0.552921,0.145261,0.138754,0.143537,0.556207,0.136811,0.133065,0.129864,0.127386,0.132757,0.127862,0.147126,0.550559,0.148018,0.540736,0.131348,0.131496,0.134551,0.139989,0.54064,0.141824,0.132245,0.137366,0.157434],
"G":[0.290505,0.285286,0.276869,0.272243,0.27844,0.272174,0.282565,0.297718,0.58827,0.174806,0.16106,0.168924,0.583945,0.173419,0.174542,0.590603,0.583827,0.172221,0.159932,0.167123,0.577926,0.169892,0.180362,0.574324,0.172539,0.159606,0.1588,0.164682,0.173275,0.174395,0.572784,0.17539,0.173341,0.175547,0.57753,0.184419,0.604527,0.166027],
"N":[0.01469,0.014708,0.014827,0.014934,0.015115,0.015185,0.015268,0.015333,0.015395,0.015416,0.015407,0.015408,0.015437,0.015431,0.015424,0.015408,0.015409,0.015434,0.015396,0.015415,0.01538,0.015409,0.015426,0.015446,0.015466,0.015483,0.015453,0.015469,0.015484,0.015512,0.015557,0.015569,0.015576,0.015546,0.015546,0.000932911,0.000975171,0.00120431],
"GC":[0.476249,0.535089,0.545412,0.542237,0.551935,0.558124,0.524196,0.517196,0.723952,0.30405,0.301223,0.723275,0.721389,0.311057,0.727463,0.735864,0.722581,0.315758,0.716139,0.303934,0.710991,0.299756,0.307748,0.707081,0.300401,0.306732,0.709359,0.3127,0.714011,0.305743,0.70428,0.309941,0.31333,0.716187,0.719354,0.316664,0.741893,0.323461]
},
"kmer_count": {
"AAAAA":144484, "AAAAT":41966, "AAAAC":29408, "AAAAG":30579, "AAATA":34195, "AAATT":26346, "AAATC":20102, "AAATG":25950, "AAACA":28214, "AAACT":23063, "AAACC":18676, "AAACG":11668, "AAAGA":36542, "AAAGT":23736, "AAAGC":19180, "AAAGG":26112,
"AATAA":29042, "AATAT":19418, "AATAC":13874, "AATAG":15272, "AATTA":19776, "AATTT":22841, "AATTC":43828, "AATTG":15119, "AATCA":15966, "AATCT":16819, "AATCC":16341, "AATCG":8257, "AATGA":25097, "AATGT":16807, "AATGC":14720, "AATGG":21075,
"AACAA":28818, "AACAT":19558, "AACAC":21331, "AACAG":18072, "AACTA":17082, "AACTT":17610, "AACTC":478972, "AACTG":19626, "AACCA":20754, "AACCT":20108, "AACCC":17788, "AACCG":7868, "AACGA":19520, "AACGT":455928, "AACGC":8679, "AACGG":10109,
"AAGAA":34505, "AAGAT":56306, "AAGAC":14603, "AAGAG":20627, "AAGTA":14735, "AAGTT":14634, "AAGTC":12450, "AAGTG":24529, "AAGCA":20377, "AAGCT":18340, "AAGCC":18960, "AAGCG":15372, "AAGGA":32982, "AAGGT":16410, "AAGGC":21495, "AAGGG":25562,
"ATAAA":29887, "ATAAT":16564, "ATAAC":13585, "ATAAG":13298, "ATATA":16168, "ATATT":16758, "ATATC":9312, "ATATG":13262, "ATACA":13990, "ATACT":11327, "ATACC":9943, "ATACG":4845, "ATAGA":19789, "ATAGT":12867, "ATAGC":10801, "ATAGG":13052,
"ATTAA":17682, "ATTAT":15122, "ATTAC":12235, "ATTAG":13620, "ATTTA":18097, "ATTTT":31976, "ATTTC":15787, "ATTTG":18503, "ATTCA":14109, "ATTCT":19452, "ATTCC":42087, "ATTCG":5973, "ATTGA":18343, "ATTGT":12522, "ATTGC":12604, "ATTGG":14767,
"ATCAA":13574, "ATCAT":13972, "ATCAC":13852, "ATCAG":13163, "ATCTA":10128, "ATCTT":15752, "ATCTC":20782, "ATCTG":18000, "ATCCA":13674, "ATCCT":16667, "ATCCC":15830, "ATCCG":6392, "ATCGA":15635, "ATCGT":542380, "ATCGC":7831, "ATCGG":8802,
"ATGAA":20171, "ATGAT":43413, "ATGAC":8922, "ATGAG":14408, "ATGTA":12627, "ATGTT":15273, "ATGTC":9768, "ATGTG":17801, "ATGCA":12654, "ATGCT":14772, "ATGCC":16021, "ATGCG":10654, "ATGGA":25691, "ATGGT":16030, "ATGGC":20975, "ATGGG":22150,
"ACAAA":29661, "ACAAT":14955, "ACAAC":17578, "ACAAG":17948, "ACATA":13881, "ACATT":15026, "ACATC":12294, "ACATG":18400, "ACACA":23204, "ACACT":18952, "ACACC":15764, "ACACG":10563, "ACAGA":26210, "ACAGT":18081, "ACAGC":17191, "ACAGG":22974,
"ACTAA":14398, "ACTAT":13566, "ACTAC":11620, "ACTAG":13307, "ACTTA":12049, "ACTTT":19765, "ACTTC":13895, "ACTTG":19480, "ACTCA":20479, "ACTCT":474997, "ACTCC":18489, "ACTCG":12108, "ACTGA":31461, "ACTGT":501830, "ACTGC":19241, "ACTGG":21126,
"ACCAA":17631, "ACCAT":16658, "ACCAC":24372, "ACCAG":20007, "ACCTA":11027, "ACCTT":14985, "ACCTC":22339, "ACCTG":26926, "ACCCA":20332, "ACCCT":18523, "ACCCC":21183, "ACCCG":33538, "ACCGA":11391, "ACCGT":5875, "ACCGC":11227, "ACCGG":10457,
"ACGAA":6533, "ACGAT":32428, "ACGAC":6008, "ACGAG":11228, "ACGTA":5034, "ACGTT":5024, "ACGTC":8464, "ACGTG":454222, "ACGCA":8560, "ACGCT":7954, "ACGCC":12704, "ACGCG":11123, "ACGGA":17532, "ACGGT":6356, "ACGGC":9578, "ACGGG":15203,
"AGAAA":34811, "AGAAT":47405, "AGAAC":496183, "AGAAG":26114, "AGATA":13075, "AGATT":13456, "AGATC":142833, "AGATG":20030, "AGACA":17801, "AGACT":14556, "AGACC":16518, "AGACG":10848, "AGAGA":28114, "AGAGT":15215, "AGAGC":21363, "AGAGG":31025,
"AGTAA":13148, "AGTAT":10798, "AGTAC":9212, "AGTAG":21211, "AGTTA":10454, "AGTTT":17371, "AGTTC":12673, "AGTTG":15031, "AGTCA":12538, "AGTCT":15214, "AGTCC":13646, "AGTCG":10460, "AGTGA":23819, "AGTGT":17235, "AGTGC":19604, "AGTGG":27459,
"AGCAA":18677, "AGCAT":15375, "AGCAC":18872, "AGCAG":28416, "AGCTA":15518, "AGCTT":15962, "AGCTC":19558, "AGCTG":35001, "AGCCA":27263, "AGCCT":28649, "AGCCC":25098, "AGCCG":19834, "AGCGA":19442, "AGCGT":8863, "AGCGC":18993, "AGCGG":25644,
"AGGAA":27049, "AGGAT":53354, "AGGAC":17886, "AGGAG":35210, "AGGTA":13563, "AGGTT":15994, "AGGTC":14255, "AGGTG":31386, "AGGCA":27981, "AGGCT":32224, "AGGCC":27173, "AGGCG":27600, "AGGGA":34585, "AGGGT":19220, "AGGGC":27068, "AGGGG":33235,
"TAAAA":35765, "TAAAT":19978, "TAAAC":14741, "TAAAG":17503, "TAATA":14160, "TAATT":16385, "TAATC":12247, "TAATG":13524, "TAACA":13962, "TAACT":12178, "TAACC":11011, "TAACG":6945, "TAAGA":21010, "TAAGT":10696, "TAAGC":11047, "TAAGG":14546,
"TATAA":15923, "TATAT":14329, "TATAC":8229, "TATAG":11206, "TATTA":13082, "TATTT":23598, "TATTC":10683, "TATTG":12148, "TATCA":9550, "TATCT":11282, "TATCC":8123, "TATCG":5489, "TATGA":17899, "TATGT":11193, "TATGC":8471, "TATGG":11673,
"TACAA":15459, "TACAT":10976, "TACAC":9495, "TACAG":14922, "TACTA":10237, "TACTT":13192, "TACTC":10826, "TACTG":12531, "TACCA":13386, "TACCT":12619, "TACCC":10580, "TACCG":5625, "TACGA":8716, "TACGT":3521, "TACGC":4513, "TACGG":5276,
"TAGAA":486704, "TAGAT":50743, "TAGAC":9056, "TAGAG":15013, "TAGTA":10696, "TAGTT":10449, "TAGTC":10523, "TAGTG":13167, "TAGCA":12903, "TAGCT":16527, "TAGCC":13996, "TAGCG":10166, "TAGGA":24174, "TAGGT":11336, "TAGGC":12723, "TAGGG":16871,
"TTAAA":24791, "TTAAT":14839, "TTAAC":10950, "TTAAG":14884, "TTATA":12889, "TTATT":20219, "TTATC":9461, "TTATG":12478, "TTACA":14145, "TTACT":12139, "TTACC":9976, "TTACG":4560, "TTAGA":18793, "TTAGT":11845, "TTAGC":12834, "TTAGG":14132,
"TTTAA":22090, "TTTAT":20901, "TTTAC":11268, "TTTAG":16069, "TTTTA":25993, "TTTTT":60972, "TTTTC":24680, "TTTTG":29302, "TTTCA":18908, "TTTCT":28255, "TTTCC":19687, "TTTCG":7174, "TTTGA":25543, "TTTGT":23016, "TTTGC":16130, "TTTGG":24354,
"TTCAA":15176, "TTCAT":14683, "TTCAC":13714, "TTCAG":17777, "TTCTA":14080, "TTCTT":23680, "TTCTC":21945, "TTCTG":23295, "TTCCA":45751, "TTCCT":22879, "TTCCC":20434, "TTCCG":9444, "TTCGA":8778, "TTCGT":6114, "TTCGC":6978, "TTCGG":9940,
"TTGAA":18355, "TTGAT":45462, "TTGAC":8681, "TTGAG":19065, "TTGTA":12727, "TTGTT":19727, "TTGTC":11709, "TTGTG":18936, "TTGCA":14867, "TTGCT":19533, "TTGCC":18106, "TTGCG":9890, "TTGGA":25599, "TTGGT":16045, "TTGGC":26079, "TTGGG":29382,
"TCAAA":18353, "TCAAT":9679, "TCAAC":12756, "TCAAG":17624, "TCATA":10834, "TCATT":14871, "TCATC":13295, "TCATG":15829, "TCACA":15955, "TCACT":18164, "TCACC":17458, "TCACG":9679, "TCAGA":23524, "TCAGT":14863, "TCAGC":18975, "TCAGG":22473,
"TCTAA":12056, "TCTAT":9173, "TCTAC":12029, "TCTAG":13691, "TCTTA":13304, "TCTTT":22567, "TCTTC":17909, "TCTTG":21402, "TCTCA":20348, "TCTCT":24309, "TCTCC":23147, "TCTCG":16985, "TCTGA":475138, "TCTGT":20211, "TCTGC":19048, "TCTGG":26603,
"TCCAA":22689, "TCCAT":19579, "TCCAC":22221, "TCCAG":30271, "TCCTA":12565, "TCCTT":17927, "TCCTC":23859, "TCCTG":31538, "TCCCA":25760, "TCCCT":22421, "TCCCC":24363, "TCCCG":18320, "TCCGA":9469, "TCCGT":6532, "TCCGC":12919, "TCCGG":14376,
"TCGAA":5924, "TCGAT":31835, "TCGAC":7635, "TCGAG":6851, "TCGTA":6275, "TCGTT":6663, "TCGTC":537241, "TCGTG":9662, "TCGCA":7552, "TCGCT":12966, "TCGCC":14597, "TCGCG":11833, "TCGGA":525350, "TCGGT":7960, "TCGGC":14622, "TCGGG":19774,
"TGAAA":24321, "TGAAT":14532, "TGAAC":469207, "TGAAG":24172, "TGATA":10338, "TGATT":13043, "TGATC":156719, "TGATG":14406, "TGACA":12265, "TGACT":12871, "TGACC":12689, "TGACG":6558, "TGAGA":25142, "TGAGT":15122, "TGAGC":21379, "TGAGG":29725,
"TGTAA":15457, "TGTAT":13675, "TGTAC":9233, "TGTAG":492200, "TGTTA":11708, "TGTTT":22400, "TGTTC":12383, "TGTTG":21040, "TGTCA":13672, "TGTCT":17210, "TGTCC":13547, "TGTCG":9491, "TGTGA":25390, "TGTGT":22935, "TGTGC":17483, "TGTGG":29891,
"TGCAA":15014, "TGCAT":13369, "TGCAC":16269, "TGCAG":26314, "TGCTA":11770, "TGCTT":19227, "TGCTC":17846, "TGCTG":33514, "TGCCA":21421, "TGCCT":29200, "TGCCC":25386, "TGCCG":14064, "TGCGA":16927, "TGCGT":7591, "TGCGC":16293, "TGCGG":19505,
"TGGAA":21938, "TGGAT":58712, "TGGAC":13925, "TGGAG":32296, "TGGTA":12989, "TGGTT":16961, "TGGTC":14221, "TGGTG":33546, "TGGCA":32232, "TGGCT":28035, "TGGCC":27238, "TGGCG":25986, "TGGGA":47654, "TGGGT":23316, "TGGGC":34607, "TGGGG":51519,
"CAAAA":30778, "CAAAT":18999, "CAAAC":16397, "CAAAG":24929, "CAATA":12939, "CAATT":11902, "CAATC":9848, "CAATG":17016, "CAACA":19853, "CAACT":15200, "CAACC":15639, "CAACG":11713, "CAAGA":32613, "CAAGT":14317, "CAAGC":17737, "CAAGG":22974,
"CATAA":13804, "CATAT":11895, "CATAC":9191, "CATAG":13990, "CATTA":11124, "CATTT":20116, "CATTC":12744, "CATTG":16368, "CATCA":13796, "CATCT":16488, "CATCC":13844, "CATCG":11363, "CATGA":25256, "CATGT":15346, "CATGC":16680, "CATGG":25409,
"CACAA":19972, "CACAT":16497, "CACAC":22796, "CACAG":24247, "CACTA":13222, "CACTT":18160, "CACTC":18355, "CACTG":32387, "CACCA":26053, "CACCT":24879, "CACCC":43667, "CACCG":14124, "CACGA":17513, "CACGT":7582, "CACGC":14114, "CACGG":14190,
"CAGAA":27364, "CAGAT":44774, "CAGAC":14725, "CAGAG":26086, "CAGTA":15799, "CAGTT":14343, "CAGTC":12840, "CAGTG":25398, "CAGCA":24473, "CAGCT":25164, "CAGCC":32868, "CAGCG":20972, "CAGGA":38895, "CAGGT":21356, "CAGGC":33821, "CAGGG":32654,
"CTAAA":16867, "CTAAT":11421, "CTAAC":10215, "CTAAG":14513, "CTATA":9905, "CTATT":10352, "CTATC":8389, "CTATG":12157, "CTACA":11399, "CTACT":13513, "CTACC":13186, "CTACG":6518, "CTAGA":25801, "CTAGT":8946, "CTAGC":12681, "CTAGG":17817,
"CTTAA":13732, "CTTAT":9928, "CTTAC":9538, "CTTAG":14233, "CTTTA":13262, "CTTTT":23679, "CTTTC":17284, "CTTTG":23566, "CTTCA":14519, "CTTCT":21017, "CTTCC":22333, "CTTCG":10417, "CTTGA":28851, "CTTGT":14784, "CTTGC":16771, "CTTGG":31941,
"CTCAA":19159, "CTCAT":15460, "CTCAC":21267, "CTCAG":30998, "CTCTA":14618, "CTCTT":20935, "CTCTC":24583, "CTCTG":485513, "CTCCA":21979, "CTCCT":31252, "CTCCC":36017, "CTCCG":16441, "CTCGA":18862, "CTCGT":8477, "CTCGC":17156, "CTCGG":23080,
"CTGAA":474969, "CTGAT":63544, "CTGAC":14219, "CTGAG":30506, "CTGTA":485196, "CTGTT":16720, "CTGTC":18520, "CTGTG":30020, "CTGCA":23205, "CTGCT":26450, "CTGCC":33331, "CTGCG":22352, "CTGGA":42266, "CTGGT":20479, "CTGGC":28242, "CTGGG":56393,
"CCAAA":23564, "CCAAT":13976, "CCAAC":16729, "CCAAG":28028, "CCATA":12688, "CCATT":16964, "CCATC":17603, "CCATG":26951, "CCACA":22590, "CCACT":24119, "CCACC":35529, "CCACG":16219, "CCAGA":33512, "CCAGT":16617, "CCAGC":35182, "CCAGG":44487,
"CCTAA":12604, "CCTAT":9065, "CCTAC":10059, "CCTAG":18930, "CCTTA":10949, "CCTTT":18851, "CCTTC":18265, "CCTTG":28086, "CCTCA":23627, "CCTCT":25981, "CCTCC":39791, "CCTCG":22434, "CCTGA":40004, "CCTGT":23234, "CCTGC":29170, "CCTGG":45845,
"CCCAA":21475, "CCCAT":17419, "CCCAC":24631, "CCCAG":43081, "CCCTA":13021, "CCCTT":20057, "CCCTC":27609, "CCCTG":37908, "CCCCA":29578, "CCCCT":27947, "CCCCC":33446, "CCCCG":26916, "CCCGA":40594, "CCCGT":9701, "CCCGC":25536, "CCCGG":30954,
"CCGAA":8112, "CCGAT":27287, "CCGAC":8455, "CCGAG":39957, "CCGTA":4284, "CCGTT":5544, "CCGTC":9547, "CCGTG":12875, "CCGCA":11481, "CCGCT":14521, "CCGCC":31892, "CCGCG":25505, "CCGGA":19515, "CCGGT":9665, "CCGGC":23388, "CCGGG":36482,
"CGAAA":6599, "CGAAT":5102, "CGAAC":7480, "CGAAG":9217, "CGATA":3224, "CGATT":3987, "CGATC":125904, "CGATG":6083, "CGACA":5475, "CGACT":6628, "CGACC":7864, "CGACG":10368, "CGAGA":39391, "CGAGT":10070, "CGAGC":12549, "CGAGG":17717,
"CGTAA":3963, "CGTAT":3720, "CGTAC":4630, "CGTAG":8584, "CGTTA":3478, "CGTTT":5739, "CGTTC":6542, "CGTTG":8596, "CGTCA":5439, "CGTCT":8598, "CGTCC":10725, "CGTCG":536775, "CGTGA":13893, "CGTGT":441413, "CGTGC":11462, "CGTGG":19487,
"CGCAA":7677, "CGCAT":6306, "CGCAC":13616, "CGCAG":17264, "CGCTA":6760, "CGCTT":10521, "CGCTC":14783, "CGCTG":24804, "CGCCA":16927, "CGCCT":21105, "CGCCC":27555, "CGCCG":28845, "CGCGA":17133, "CGCGT":8505, "CGCGC":26433, "CGCGG":34746,
"CGGAA":11680, "CGGAT":36972, "CGGAC":524782, "CGGAG":22495, "CGGTA":5664, "CGGTT":6823, "CGGTC":9780, "CGGTG":18619, "CGGCA":12751, "CGGCT":18403, "CGGCC":27257, "CGGCG":35972, "CGGGA":32334, "CGGGT":16928, "CGGGC":34426, "CGGGG":42861,
"GAAAA":28339, "GAAAT":22224, "GAAAC":17802, "GAAAG":26091, "GAATA":12092, "GAATT":42685, "GAATC":12536, "GAATG":18029, "GAACA":20902, "GAACT":482442, "GAACC":17777, "GAACG":464115, "GAAGA":31870, "GAAGT":15608, "GAAGC":20720, "GAAGG":28769,
"GATAA":11243, "GATAT":8100, "GATAC":6900, "GATAG":12579, "GATTA":11030, "GATTT":13539, "GATTC":10523, "GATTG":11687, "GATCA":12332, "GATCT":20365, "GATCC":12448, "GATCG":551311, "GATGA":16077, "GATGT":10792, "GATGC":11066, "GATGG":22922,
"GACAA":11935, "GACAT":10538, "GACAC":11824, "GACAG":23459, "GACTA":9618, "GACTT":12878, "GACTC":16779, "GACTG":512459, "GACCA":13961, "GACCT":14418, "GACCC":16237, "GACCG":9650, "GACGA":9157, "GACGT":6081, "GACGC":10169, "GACGG":17273,
"GAGAA":51564, "GAGAT":34097, "GAGAC":19581, "GAGAG":28918, "GAGTA":10263, "GAGTT":14000, "GAGTC":14195, "GAGTG":21729, "GAGCA":17611, "GAGCT":21426, "GAGCC":29376, "GAGCG":21901, "GAGGA":33883, "GAGGT":23792, "GAGGC":41912, "GAGGG":35618,
"GTAAA":13870, "GTAAT":12639, "GTAAC":8367, "GTAAG":12070, "GTATA":9086, "GTATT":11476, "GTATC":6684, "GTATG":10288, "GTACA":9535, "GTACT":8800, "GTACC":7805, "GTACG":5421, "GTAGA":499569, "GTAGT":10927, "GTAGC":14928, "GTAGG":18502,
"GTTAA":9963, "GTTAT":8381, "GTTAC":7267, "GTTAG":11241, "GTTTA":10542, "GTTTT":21786, "GTTTC":15355, "GTTTG":16413, "GTTCA":11950, "GTTCT":13584, "GTTCC":12937, "GTTCG":7459, "GTTGA":16891, "GTTGT":12445, "GTTGC":14921, "GTTGG":21693,
"GTCAA":8590, "GTCAT":10032, "GTCAC":11620, "GTCAG":15974, "GTCTA":6879, "GTCTT":14125, "GTCTC":19044, "GTCTG":17605, "GTCCA":10073, "GTCCT":14016, "GTCCC":17661, "GTCCG":10873, "GTCGA":8143, "GTCGT":7433, "GTCGC":13387, "GTCGG":540040,
"GTGAA":16778, "GTGAT":42240, "GTGAC":12400, "GTGAG":24846, "GTGTA":18142, "GTGTT":15422, "GTGTC":13707, "GTGTG":27617, "GTGCA":16964, "GTGCT":19439, "GTGCC":19810, "GTGCG":15220, "GTGGA":31188, "GTGGT":23955, "GTGGC":31938, "GTGGG":45251,
"GCAAA":16852, "GCAAT":12398, "GCAAC":14332, "GCAAG":21205, "GCATA":9940, "GCATT":12753, "GCATC":11716, "GCATG":21074, "GCACA":18789, "GCACT":19802, "GCACC":37336, "GCACG":15962, "GCAGA":28589, "GCAGT":19226, "GCAGC":31695, "GCAGG":37819,
"GCTAA":12663, "GCTAT":8957, "GCTAC":11088, "GCTAG":17326, "GCTTA":10006, "GCTTT":16609, "GCTTC":17957, "GCTTG":22702, "GCTCA":20075, "GCTCT":21409, "GCTCC":23657, "GCTCG":18296, "GCTGA":36860, "GCTGT":24422, "GCTGC":37634, "GCTGG":53769,
"GCCAA":18324, "GCCAT":19780, "GCCAC":25653, "GCCAG":34002, "GCCTA":12605, "GCCTT":19693, "GCCTC":37066, "GCCTG":41948, "GCCCA":26858, "GCCCT":27215, "GCCCC":35640, "GCCCG":26600, "GCCGA":20034, "GCCGT":9793, "GCCGC":32741, "GCCGG":33450,
"GCGAA":7034, "GCGAT":48678, "GCGAC":8299, "GCGAG":18556, "GCGTA":4535, "GCGTT":6984, "GCGTC":10868, "GCGTG":20100, "GCGCA":14680, "GCGCT":19659, "GCGCC":31402, "GCGCG":36411, "GCGGA":32288, "GCGGT":16489, "GCGGC":45598, "GCGGG":53076,
"GGAAA":25299, "GGAAT":17488, "GGAAC":15925, "GGAAG":32817, "GGATA":10515, "GGATT":15431, "GGATC":171012, "GGATG":19170, "GGACA":19682, "GGACT":522209, "GGACC":16086, "GGACG":14159, "GGAGA":37217, "GGAGT":22386, "GGAGC":33141, "GGAGG":53899,
"GGTAA":12198, "GGTAT":9070, "GGTAC":7691, "GGTAG":18427, "GGTTA":9300, "GGTTT":17854, "GGTTC":14222, "GGTTG":19222, "GGTCA":12380, "GGTCT":15849, "GGTCC":14689, "GGTCG":15766, "GGTGA":30707, "GGTGT":20157, "GGTGC":21795, "GGTGG":49925,
"GGCAA":19231, "GGCAT":17746, "GGCAC":36519, "GGCAG":40897, "GGCTA":12960, "GGCTT":18733, "GGCTC":28693, "GGCTG":56521, "GGCCA":28442, "GGCCT":27986, "GGCCC":34082, "GGCCG":32329, "GGCGA":27323, "GGCGT":15620, "GGCGC":35073, "GGCGG":64595,
"GGGAA":28548, "GGGAT":67168, "GGGAC":21828, "GGGAG":53607, "GGGTA":12539, "GGGTT":20153, "GGGTC":20951, "GGGTG":38420, "GGGCA":29542, "GGGCT":33928, "GGGCC":37599, "GGGCG":49766, "GGGGA":52154, "GGGGT":31489, "GGGGC":49488, "GGGGG":530960
},
"overrepresented_sequences": {
"AAAAAAAAAAAAAAAAAAAA":78,
"AAAGATCGTCGGACTGTAGA":233,
"AACGATCGTCGGACTGTAGA":304,
"AAGGATCGTCGGACTGTAGA":292,
"AATGATCGTCGGACTGTAGA":322,
"ACAGATCGTCGGACTGTAGA":183,
"ACCGATCGTCGGACTGTAGA":203,
"ACCTGATCGTCGGACTGTAG":144,
"ACGGATCGTCGGACTGTAGA":198,
"ACTGATCGTCGGACTGTAGA":162,
"ACTGTAGAACTCTGAACGTG":18,
"AGATCGTCGGACTGTAGAAC":38,
"AGCGATCGTCGGACTGTAGA":427,
"AGCTGATCGTCGGACTGTAG":152,
"AGGGATCGTCGGACTGTAGA":291,
"AGTGATCGTCGGACTGTAGA":232,
"ATAGATCGTCGGACTGTAGA":294,
"ATCGATCGTCGGACTGTAGA":188,
"ATCGTCGGACTGTAGAACTC":36,
"ATGATCGTCGGACTGTAGAA":17,
"ATGGATCGTCGGACTGTAGA":204,
"ATTGATCGTCGGACTGTAGA":306,
"CAAAGATCGTCGGACTGTAG":136,
"CAAGATCGTCGGACTGTAGA":191,
"CAAGGATCGTCGGACTGTAG":138,
"CACGATCGTCGGACTGTAGA":248,
"CACTGATCGTCGGACTGTAG":182,
"CAGGATCGTCGGACTGTAGA":105,
"CATCGATCGTCGGACTGTAG":120,
"CATGATCGTCGGACTGTAGA":191,
"CATGGATCGTCGGACTGTAG":132,
"CCAAGATCGTCGGACTGTAG":225,
"CCAGATCGTCGGACTGTAGA":137,
"CCAGGATCGTCGGACTGTAG":183,
"CCATGATCGTCGGACTGTAG":187,
"CCCAGATCGTCGGACTGTAG":212,
"CCCCTGATCGTCGGACTGTA":138,
"CCCGATCGTCGGACTGTAGA":334,
"CCCGGATCGTCGGACTGTAG":115,
"CCCTGATCGTCGGACTGTAG":251,
"CCGATCGTCGGACTGTAGAA":92,
"CCGGATCGTCGGACTGTAGA":108,
"CCTAGATCGTCGGACTGTAG":178,
"CCTCGATCGTCGGACTGTAG":171,
"CCTGGATCGTCGGACTGTAG":256,
"CCTTGATCGTCGGACTGTAG":195,
"CGAGATCGTCGGACTGTAGA":136,
"CGATCGTCGGACTGTAGAAC":26,
"CGCGATCGTCGGACTGTAGA":212,
"CGCGGATCGTCGGACTGTAG":115,
"CGCTGATCGTCGGACTGTAG":134,
"CGGACTGTAGAACTCTGAAC":77,
"CGGGATCGTCGGACTGTAGA":243,
"CGGGGATCGTCGGACTGTAG":125,
"CGTCGGACTGTAGAACTCTG":68,
"CGTGATCGTCGGACTGTAGA":250,
"CTAGATCGTCGGACTGTAGA":168,
"CTAGGATCGTCGGACTGTAG":113,
"CTCGATCGTCGGACTGTAGA":322,
"CTCTGATCGTCGGACTGTAG":144,
"CTGCGATCGTCGGACTGTAG":124,
"CTGGATCGTCGGACTGTAGA":162,
"CTGGGATCGTCGGACTGTAG":227,
"CTGTGATCGTCGGACTGTAG":134,
"CTTGATCGTCGGACTGTAGA":205,
"CTTGGCACCCGAGAATTCCA":146,
"CTTTGATCGTCGGACTGTAG":123,
"GAAGATCGTCGGACTGTAGA":264,
"GACGATCGTCGGACTGTAGA":186,
"GACTGTAGAACTCTGAACGT":20,
"GAGATCGTCGGACTGTAGAA":95,
"GAGGATCGTCGGACTGTAGA":116,
"GATCGTCGGACTGTAGAACT":140,
"GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT":247,
"GATGATCGTCGGACTGTAGA":189,
"GCAAGATCGTCGGACTGTAG":157,
"GCACGATCGTCGGACTGTAG":149,
"GCAGATCGTCGGACTGTAGA":194,
"GCAGGATCGTCGGACTGTAG":165,
"GCATGATCGTCGGACTGTAG":149,
"GCCAGATCGTCGGACTGTAG":195,
"GCCGATCGTCGGACTGTAGA":255,
"GCCTGATCGTCGGACTGTAG":198,
"GCGCGATCGTCGGACTGTAG":167,
"GCGGATCGTCGGACTGTAGA":214,
"GCGGGATCGTCGGACTGTAG":231,
"GCTAGATCGTCGGACTGTAG":148,
"GCTGGATCGTCGGACTGTAG":227,
"GCTTGATCGTCGGACTGTAG":142,
"GGACTGTAGAACTCTGAACG":77,
"GGAGATCGTCGGACTGTAGA":161,
"GGAGGATCGTCGGACTGTAG":136,
"GGATCGTCGGACTGTAGAAC":19,
"GGCAGATCGTCGGACTGTAG":150,
"GGCGATCGTCGGACTGTAGA":267,
"GGCGGATCGTCGGACTGTAG":210,
"GGCTGATCGTCGGACTGTAG":193,
"GGGAGATCGTCGGACTGTAG":164,
"GGGCGATCGTCGGACTGTAG":241,
"GGGGATCGTCGGACTGTAGA":111,
"GGGGGATCGTCGGACTGTAG":273,
"GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG":607,
"GGTGATCGTCGGACTGTAGA":358,
"GGTGGATCGTCGGACTGTAG":163,
"GTAGATCGTCGGACTGTAGA":294,
"GTCGATCGTCGGACTGTAGA":151,
"GTCGGACTGTAGAACTCTGA":90,
"GTGGATCGTCGGACTGTAGA":224,
"GTGGGATCGTCGGACTGTAG":135,
"GTTGATCGTCGGACTGTAGA":232,
"NNNNNNNNNNNNNNNNNNNN":829,
"TAAGATCGTCGGACTGTAGA":318,
"TACGATCGTCGGACTGTAGA":217,
"TAGATCGTCGGACTGTAGAA":12,
"TAGGATCGTCGGACTGTAGA":222,
"TATGATCGTCGGACTGTAGA":234,
"TCAGATCGTCGGACTGTAGA":147,
"TCCTGATCGTCGGACTGTAG":146,
"TCGGACTGTAGAACTCTGAA":85,
"TCGGATCGTCGGACTGTAGA":211,
"TCGTCGGACTGTAGAACTCT":51,
"TCTGATCGTCGGACTGTAGA":171,
"TGAGATCGTCGGACTGTAGA":201,
"TGATCGTCGGACTGTAGAAC":30,
"TGCGATCGTCGGACTGTAGA":264,
"TGCTGATCGTCGGACTGTAG":149,
"TGGGATCGTCGGACTGTAGA":195,
"TGGGGATCGTCGGACTGTAG":181,
"TGTGATCGTCGGACTGTAGA":216,
"TTAGATCGTCGGACTGTAGA":258,
"TTCGATCGTCGGACTGTAGA":189,
"TTGGATCGTCGGACTGTAGA":318,
"TTTGATCGTCGGACTGTAGA":146 }
},
"read1_after_filtering": {
"total_reads": 439519,
"total_bases": 16134044,
"q20_bases": 15454671,
"q30_bases": 15178418,
"total_cycles": 38,
"quality_curves": {
"A":[30.6532,30.5453,30.8913,30.8849,30.9311,34.3902,34.3326,34.2759,34.6565,33.5438,33.4735,33.5068,34.5057,33.0241,33.5308,34.9244,34.8535,33.9026,33.836,33.0547,34.616,33.1939,32.4045,34.6388,33.0761,33.7631,33.4267,33.0139,32.9276,32.7339,34.3503,32.8285,33.5863,33.5355,34.4883,32.7721,34.1664,34.5141],
"T":[31.3078,31.1954,31.2971,31.318,31.3362,35.0028,35.0142,35.0109,35.2502,35.2586,34.5057,35.0355,35.1825,34.3324,34.9186,35.2775,35.2669,35.2803,35.1731,34.3213,35.1984,34.2713,35.1441,35.2433,35.1893,35.183,35.0639,34.2394,34.7854,33.9934,35.1507,35.2173,35.1709,35.0959,35.1152,34.2133,35.1085,33.9155],
"C":[31.3065,31.2402,31.3489,31.3552,31.3797,35.0545,35.0538,35.0951,35.3558,35.2595,35.2501,34.3734,35.2926,35.1488,34.1112,35.2777,35.3457,35.338,34.4882,35.0701,35.3257,35.1122,35.2467,35.3024,35.1783,35.2334,34.4872,34.9965,33.8475,34.8599,35.2382,35.157,35.2116,34.5276,35.1893,35.0442,35.2739,34.8959],
"G":[31.0271,30.9235,31.1206,31.1744,31.1499,34.778,34.7776,34.767,34.2991,34.8659,35.0226,35.0349,34.1258,34.9288,34.9755,34.3316,34.3558,34.9292,35.1251,34.9157,34.2145,34.8996,34.7812,34.3093,34.8709,34.882,35.012,34.8599,34.7999,34.6789,33.9708,34.788,34.82,34.9695,34.027,34.7688,34.0838,34.7812],
"mean":[30.9883,30.9649,31.1654,31.189,31.2038,34.8159,34.7988,34.7871,34.8567,34.7021,34.5837,34.5052,34.732,34.3846,34.3951,34.9203,34.9251,34.8506,34.6568,34.3723,34.8104,34.4034,34.393,34.8491,34.5659,34.7538,34.4988,34.3057,34.105,34.1008,34.6506,34.4881,34.6932,34.5402,34.6847,34.2513,34.6906,34.5141]
},
"content_curves": {
"A":[0.369968,0.266667,0.243432,0.231772,0.231583,0.228707,0.232379,0.240816,0.222382,0.261274,0.233696,0.231985,0.233023,0.231692,0.229876,0.223333,0.223679,0.251853,0.234267,0.233034,0.236363,0.235403,0.246242,0.236588,0.250974,0.253052,0.243299,0.239327,0.236056,0.236394,0.233299,0.248975,0.246062,0.235566,0.22024,0.223857,0.179453,0],
"T":[0.207816,0.261224,0.266491,0.263752,0.249741,0.241496,0.229412,0.235335,0.22748,0.226129,0.254091,0.224052,0.233546,0.259008,0.229471,0.231046,0.228532,0.223046,0.219397,0.248556,0.228104,0.235906,0.225647,0.224771,0.22549,0.225194,0.223146,0.235225,0.225901,0.236901,0.233142,0.227017,0.22854,0.230398,0.234023,0.248826,0.265889,0.349438],
"C":[0.148023,0.225119,0.234859,0.242479,0.252608,0.261572,0.254742,0.246699,0.247864,0.237278,0.239373,0.26491,0.232438,0.235885,0.272118,0.251607,0.252492,0.249466,0.277665,0.249682,0.248986,0.252754,0.247628,0.253903,0.242044,0.249389,0.263877,0.251336,0.260841,0.252284,0.250995,0.250514,0.253489,0.267365,0.260156,0.25354,0.269998,0.362529],
"G":[0.274186,0.24699,0.255218,0.261998,0.266068,0.268225,0.283467,0.277151,0.302269,0.275301,0.272839,0.279051,0.300981,0.273406,0.268534,0.294005,0.295293,0.275626,0.268671,0.268712,0.286545,0.275931,0.280479,0.284732,0.281469,0.272352,0.269659,0.274086,0.277164,0.274372,0.282503,0.273437,0.271825,0.266595,0.285551,0.273712,0.284603,0.287846],
"N":[6.82564e-06,0,0,0,0,0,0,0,4.55043e-06,1.82017e-05,0,2.27521e-06,1.13761e-05,9.10086e-06,0,9.10086e-06,4.55043e-06,9.10086e-06,0,1.59265e-05,2.27521e-06,6.82564e-06,4.55043e-06,6.82564e-06,2.27521e-05,1.36513e-05,1.84418e-05,2.57565e-05,3.82753e-05,4.85115e-05,6.13363e-05,5.7072e-05,8.2911e-05,7.59955e-05,3.03982e-05,6.47944e-05,5.57543e-05,0.000187018],
"GC":[0.422209,0.472109,0.490077,0.504476,0.518676,0.529797,0.538209,0.52385,0.550133,0.512579,0.512212,0.543961,0.533419,0.509291,0.540652,0.545612,0.547785,0.525092,0.546336,0.518394,0.535531,0.528685,0.528107,0.538634,0.523513,0.521741,0.533537,0.525423,0.538005,0.526656,0.533498,0.52395,0.525314,0.53396,0.545706,0.527253,0.554602,0.650375]
},
"kmer_count": {
"AAAAA":66178, "AAAAT":31082, "AAAAC":19402, "AAAAG":21480, "AAATA":23999, "AAATT":21294, "AAATC":13821, "AAATG":19489, "AAACA":19196, "AAACT":16973, "AAACC":14126, "AAACG":6086, "AAAGA":20768, "AAAGT":17811, "AAAGC":14513, "AAAGG":18422,
"AATAA":18974, "AATAT":14556, "AATAC":10573, "AATAG":10198, "AATTA":15204, "AATTT":19652, "AATTC":30943, "AATTG":10658, "AATCA":11846, "AATCT":12302, "AATCC":13743, "AATCG":4148, "AATGA":13965, "AATGT":12859, "AATGC":11141, "AATGG":14646,
"AACAA":17877, "AACAT":14107, "AACAC":12605, "AACAG":13790, "AACTA":10695, "AACTT":13395, "AACTC":17790, "AACTG":13290, "AACCA":15248, "AACCT":14395, "AACCC":13180, "AACCG":4700, "AACGA":5965, "AACGT":9237, "AACGC":5360, "AACGG":5503,
"AAGAA":23262, "AAGAT":14829, "AAGAC":12114, "AAGAG":17498, "AAGTA":11333, "AAGTT":12680, "AAGTC":10647, "AAGTG":18066, "AAGCA":15329, "AAGCT":13513, "AAGCC":14527, "AAGCG":8619, "AAGGA":19226, "AAGGT":13096, "AAGGC":16269, "AAGGG":17595,
"ATAAA":19526, "ATAAT":12482, "ATAAC":9130, "ATAAG":8598, "ATATA":12206, "ATATT":13982, "ATATC":6852, "ATATG":9178, "ATACA":10785, "ATACT":8813, "ATACC":7325, "ATACG":2112, "ATAGA":9411, "ATAGT":8887, "ATAGC":7494, "ATAGG":8305,
"ATTAA":13355, "ATTAT":12464, "ATTAC":9589, "ATTAG":9794, "ATTTA":15478, "ATTTT":29205, "ATTTC":14134, "ATTTG":14693, "ATTCA":11866, "ATTCT":17584, "ATTCC":29174, "ATTCG":3388, "ATTGA":9727, "ATTGT":10406, "ATTGC":9603, "ATTGG":9953,
"ATCAA":9445, "ATCAT":10679, "ATCAC":10846, "ATCAG":10175, "ATCTA":7590, "ATCTT":12950, "ATCTC":13227, "ATCTG":12549, "ATCCA":10671, "ATCCT":13260, "ATCCC":13214, "ATCCG":4775, "ATCGA":3847, "ATCGT":12862, "ATCGC":5155, "ATCGG":5211,
"ATGAA":14748, "ATGAT":11357, "ATGAC":7302, "ATGAG":11966, "ATGTA":9339, "ATGTT":13478, "ATGTC":8564, "ATGTG":13453, "ATGCA":9580, "ATGCT":10963, "ATGCC":12176, "ATGCG":4633, "ATGGA":14658, "ATGGT":12975, "ATGGC":16000, "ATGGG":14677,
"ACAAA":19315, "ACAAT":10158, "ACAAC":10776, "ACAAG":11559, "ACATA":9702, "ACATT":12145, "ACATC":8685, "ACATG":12781, "ACACA":16408, "ACACT":11989, "ACACC":11427, "ACACG":5522, "ACAGA":17382, "ACAGT":12982, "ACAGC":14182, "ACAGG":18830,
"ACTAA":10024, "ACTAT":8920, "ACTAC":8822, "ACTAG":8314, "ACTTA":9154, "ACTTT":16562, "ACTTC":11729, "ACTTG":13822, "ACTCA":13457, "ACTCT":18581, "ACTCC":15119, "ACTCG":6976, "ACTGA":15203, "ACTGT":17301, "ACTGC":15511, "ACTGG":14699,
"ACCAA":12113, "ACCAT":12393, "ACCAC":18234, "ACCAG":14361, "ACCTA":7715, "ACCTT":11513, "ACCTC":16266, "ACCTG":18347, "ACCCA":14624, "ACCCT":12751, "ACCCC":14774, "ACCCG":23831, "ACCGA":4832, "ACCGT":4250, "ACCGC":8520, "ACCGG":7277,
"ACGAA":4187, "ACGAT":4042, "ACGAC":3849, "ACGAG":6050, "ACGTA":2957, "ACGTT":3512, "ACGTC":4186, "ACGTG":11000, "ACGCA":5486, "ACGCT":4976, "ACGCC":9092, "ACGCG":6577, "ACGGA":7032, "ACGGT":4574, "ACGGC":7477, "ACGGG":8911,
"AGAAA":25438, "AGAAT":34885, "AGAAC":21303, "AGAAG":20450, "AGATA":10026, "AGATT":11866, "AGATC":13078, "AGATG":16953, "AGACA":15036, "AGACT":12529, "AGACC":14386, "AGACG":8328, "AGAGA":23034, "AGAGT":13757, "AGAGC":18857, "AGAGG":26889,
"AGTAA":10560, "AGTAT":8723, "AGTAC":7342, "AGTAG":13897, "AGTTA":8926, "AGTTT":15994, "AGTTC":11696, "AGTTG":12273, "AGTCA":10919, "AGTCT":13587, "AGTCC":12551, "AGTCG":6853, "AGTGA":16941, "AGTGT":13803, "AGTGC":16858, "AGTGG":21464,
"AGCAA":13504, "AGCAT":11500, "AGCAC":13510, "AGCAG":22948, "AGCTA":11784, "AGCTT":12752, "AGCTC":16033, "AGCTG":26248, "AGCCA":21384, "AGCCT":22960, "AGCCC":20145, "AGCCG":16397, "AGCGA":7827, "AGCGT":6106, "AGCGC":13808, "AGCGG":17719,
"AGGAA":22341, "AGGAT":15281, "AGGAC":13557, "AGGAG":30615, "AGGTA":10552, "AGGTT":13927, "AGGTC":12442, "AGGTG":24629, "AGGCA":21995, "AGGCT":27065, "AGGCC":22647, "AGGCG":20392, "AGGGA":23083, "AGGGT":16395, "AGGGC":20996, "AGGGG":22466,
"TAAAA":24421, "TAAAT":15906, "TAAAC":10335, "TAAAG":12427, "TAATA":10493, "TAATT":13759, "TAATC":9894, "TAATG":9462, "TAACA":10198, "TAACT":9088, "TAACC":8380, "TAACG":3253, "TAAGA":11026, "TAAGT":9077, "TAAGC":7759, "TAAGG":10453,
"TATAA":11307, "TATAT":11776, "TATAC":6218, "TATAG":7731, "TATTA":10506, "TATTT":21341, "TATTC":9135, "TATTG":8806, "TATCA":7389, "TATCT":9162, "TATCC":6346, "TATCG":2189, "TATGA":8636, "TATGT":9555, "TATGC":6020, "TATGG":8206,
"TACAA":11003, "TACAT":8886, "TACAC":7136, "TACAG":12482, "TACTA":7774, "TACTT":11162, "TACTC":8468, "TACTG":9152, "TACCA":10475, "TACCT":9477, "TACCC":7907, "TACCG":3621, "TACGA":2662, "TACGT":2506, "TACGC":2892, "TACGG":3302,
"TAGAA":18321, "TAGAT":8502, "TAGAC":7169, "TAGAG":12581, "TAGTA":8426, "TAGTT":9152, "TAGTC":7604, "TAGTG":9429, "TAGCA":9269, "TAGCT":12492, "TAGCC":10393, "TAGCG":4675, "TAGGA":11608, "TAGGT":8753, "TAGGC":9005, "TAGGG":11093,
"TTAAA":19911, "TTAAT":12420, "TTAAC":8276, "TTAAG":11225, "TTATA":10535, "TTATT":18031, "TTATC":7712, "TTATG":9233, "TTACA":11786, "TTACT":10051, "TTACC":7955, "TTACG":2320, "TTAGA":10371, "TTAGT":10448, "TTAGC":10160, "TTAGG":10689,
"TTTAA":19166, "TTTAT":18707, "TTTAC":9644, "TTTAG":12882, "TTTTA":23565, "TTTTT":55952, "TTTTC":23065, "TTTTG":25174, "TTTCA":17293, "TTTCT":26493, "TTTCC":18098, "TTTCG":4992, "TTTGA":16991, "TTTGT":21092, "TTTGC":13573, "TTTGG":20120,
"TTCAA":12830, "TTCAT":12970, "TTCAC":11740, "TTCAG":16082, "TTCTA":12648, "TTCTT":21961, "TTCTC":20188, "TTCTG":20450, "TTCCA":32011, "TTCCT":20481, "TTCCC":17864, "TTCCG":8045, "TTCGA":3559, "TTCGT":4723, "TTCGC":5249, "TTCGG":7671,
"TTGAA":15705, "TTGAT":11392, "TTGAC":7445, "TTGAG":16233, "TTGTA":10830, "TTGTT":17932, "TTGTC":10279, "TTGTG":14727, "TTGCA":12133, "TTGCT":15959, "TTGCC":14341, "TTGCG":5143, "TTGGA":14742, "TTGGT":13394, "TTGGC":21489, "TTGGG":21689,
"TCAAA":14203, "TCAAT":7264, "TCAAC":8919, "TCAAG":12804, "TCATA":8380, "TCATT":13191, "TCATC":10185, "TCATG":11703, "TCACA":12669, "TCACT":15245, "TCACC":14161, "TCACG":6017, "TCAGA":14967, "TCAGT":13460, "TCAGC":17032, "TCAGG":19605,
"TCTAA":9475, "TCTAT":7808, "TCTAC":10200, "TCTAG":9655, "TCTTA":11149, "TCTTT":20793, "TCTTC":15555, "TCTTG":16899, "TCTCA":17409, "TCTCT":21461, "TCTCC":20698, "TCTCG":9924, "TCTGA":18142, "TCTGT":18447, "TCTGC":16903, "TCTGG":21437,
"TCCAA":16359, "TCCAT":14610, "TCCAC":16238, "TCCAG":23361, "TCCTA":10189, "TCCTT":15705, "TCCTC":19642, "TCCTG":25158, "TCCCA":21862, "TCCCT":18022, "TCCCC":19742, "TCCCG":13960, "TCCGA":5246, "TCCGT":5605, "TCCGC":11489, "TCCGG":12589,
"TCGAA":3968, "TCGAT":3718, "TCGAC":3436, "TCGAG":5202, "TCGTA":3071, "TCGTT":4729, "TCGTC":13628, "TCGTG":6351, "TCGCA":4946, "TCGCT":10136, "TCGCC":10877, "TCGCG":7423, "TCGGA":17294, "TCGGT":6632, "TCGGC":12005, "TCGGG":13804,
"TGAAA":18630, "TGAAT":12042, "TGAAC":19197, "TGAAG":16676, "TGATA":7882, "TGATT":11635, "TGATC":14398, "TGATG":11646, "TGACA":10398, "TGACT":10989, "TGACC":10952, "TGACG":4378, "TGAGA":18385, "TGAGT":13993, "TGAGC":18405, "TGAGG":25463,
"TGTAA":12961, "TGTAT":11136, "TGTAC":7242, "TGTAG":16213, "TGTTA":10064, "TGTTT":20749, "TGTTC":11559, "TGTTG":16650, "TGTCA":12046, "TGTCT":15920, "TGTCC":12608, "TGTCG":6849, "TGTGA":15022, "TGTGT":20246, "TGTGC":14650, "TGTGG":21142,
"TGCAA":11448, "TGCAT":10311, "TGCAC":11914, "TGCAG":22628, "TGCTA":8786, "TGCTT":15837, "TGCTC":14419, "TGCTG":24729, "TGCCA":16209, "TGCCT":22857, "TGCCC":19517, "TGCCG":10090, "TGCGA":5639, "TGCGT":5499, "TGCGC":11627, "TGCGG":13537,
"TGGAA":17775, "TGGAT":14341, "TGGAC":10449, "TGGAG":27340, "TGGTA":9674, "TGGTT":14487, "TGGTC":12178, "TGGTG":25142, "TGGCA":25667, "TGGCT":22047, "TGGCC":22031, "TGGCG":18560, "TGGGA":28145, "TGGGT":19315, "TGGGC":25264, "TGGGG":32006,
"CAAAA":20513, "CAAAT":13843, "CAAAC":11484, "CAAAG":16274, "CAATA":8422, "CAATT":8895, "CAATC":6748, "CAATG":10496, "CAACA":13514, "CAACT":10299, "CAACC":10842, "CAACG":5261, "CAAGA":14737, "CAAGT":11765, "CAAGC":12619, "CAAGG":15485,
"CATAA":9377, "CATAT":8979, "CATAC":6452, "CATAG":7800, "CATTA":8110, "CATTT":17543, "CATTC":10742, "CATTG":10732, "CATCA":10260, "CATCT":13062, "CATCC":11081, "CATCG":4575, "CATGA":11190, "CATGT":12693, "CATGC":12062, "CATGG":17439,
"CACAA":12544, "CACAT":11769, "CACAC":15849, "CACAG":18124, "CACTA":9003, "CACTT":14269, "CACTC":13271, "CACTG":20585, "CACCA":18526, "CACCT":17693, "CACCC":30932, "CACCG":9077, "CACGA":5116, "CACGT":5465, "CACGC":9386, "CACGG":8492,
"CAGAA":19251, "CAGAT":12460, "CAGAC":12518, "CAGAG":23582, "CAGTA":10391, "CAGTT":12934, "CAGTC":11410, "CAGTG":21604, "CAGCA":19752, "CAGCT":21405, "CAGCC":29417, "CAGCG":14711, "CAGGA":24320, "CAGGT":18676, "CAGGC":29405, "CAGGG":27442,
"CTAAA":12254, "CTAAT":8689, "CTAAC":7558, "CTAAG":9315, "CTATA":7169, "CTATT":8464, "CTATC":5800, "CTATG":7627, "CTACA":9229, "CTACT":11376, "CTACC":10616, "CTACG":4116, "CTAGA":10367, "CTAGT":6958, "CTAGC":8790, "CTAGG":11623,
"CTTAA":10479, "CTTAT":7746, "CTTAC":7434, "CTTAG":9676, "CTTTA":10858, "CTTTT":21292, "CTTTC":15431, "CTTTG":18218, "CTTCA":12530, "CTTCT":18754, "CTTCC":19515, "CTTCG":6657, "CTTGA":13794, "CTTGT":12139, "CTTGC":12706, "CTTGG":24342,
"CTCAA":13846, "CTCAT":11841, "CTCAC":16483, "CTCAG":24063, "CTCTA":10760, "CTCTT":17027, "CTCTC":19460, "CTCTG":28216, "CTCCA":18022, "CTCCT":24788, "CTCCC":27859, "CTCCG":13475, "CTCGA":5494, "CTCGT":6019, "CTCGC":12839, "CTCGG":16886,
"CTGAA":21336, "CTGAT":11064, "CTGAC":11888, "CTGAG":26022, "CTGTA":18086, "CTGTT":14502, "CTGTC":16810, "CTGTG":22363, "CTGCA":19974, "CTGCT":21440, "CTGCC":27028, "CTGCG":16411, "CTGGA":22210, "CTGGT":16802, "CTGGC":24290, "CTGGG":42210,
"CCAAA":16638, "CCAAT":9677, "CCAAC":11691, "CCAAG":17497, "CCATA":8311, "CCATT":12924, "CCATC":12639, "CCATG":17822, "CCACA":16047, "CCACT":17507, "CCACC":25212, "CCACG":9682, "CCAGA":17125, "CCAGT":13847, "CCAGC":28836, "CCAGG":34696,
"CCTAA":9148, "CCTAT":6616, "CCTAC":8082, "CCTAG":10663, "CCTTA":7866, "CCTTT":15476, "CCTTC":15496, "CCTTG":18802, "CCTCA":18369, "CCTCT":19007, "CCTCC":30490, "CCTCG":13394, "CCTGA":17181, "CCTGT":19083, "CCTGC":23717, "CCTGG":33683,
"CCCAA":14766, "CCCAT":11817, "CCCAC":17536, "CCCAG":32080, "CCCTA":8256, "CCCTT":14269, "CCCTC":19771, "CCCTG":23004, "CCCCA":19837, "CCCCT":17361, "CCCCC":21116, "CCCCG":19164, "CCCGA":24427, "CCCGT":7837, "CCCGC":20756, "CCCGG":24345,
"CCGAA":6378, "CCGAT":4360, "CCGAC":6382, "CCGAG":30246, "CCGTA":2980, "CCGTT":4376, "CCGTC":8142, "CCGTG":9482, "CCGCA":8877, "CCGCT":11305, "CCGCC":26529, "CCGCG":21020, "CCGGA":11563, "CCGGT":8134, "CCGGC":21242, "CCGGG":28916,
"CGAAA":4511, "CGAAT":3777, "CGAAC":4775, "CGAAG":6416, "CGATA":1867, "CGATT":3180, "CGATC":7730, "CGATG":4454, "CGACA":3898, "CGACT":4689, "CGACC":6490, "CGACG":5187, "CGAGA":25624, "CGAGT":6511, "CGAGC":10696, "CGAGG":14202,
"CGTAA":2431, "CGTAT":2236, "CGTAC":2731, "CGTAG":4110, "CGTTA":2271, "CGTTT":4523, "CGTTC":5851, "CGTTG":4932, "CGTCA":3951, "CGTCT":6701, "CGTCC":8854, "CGTCG":14777, "CGTGA":6685, "CGTGT":8119, "CGTGC":8645, "CGTGG":12697,
"CGCAA":4297, "CGCAT":3776, "CGCAC":8674, "CGCAG":12701, "CGCTA":4081, "CGCTT":7951, "CGCTC":11169, "CGCTG":16593, "CGCCA":10675, "CGCCT":15080, "CGCCC":19700, "CGCCG":23592, "CGCGA":6616, "CGCGT":6402, "CGCGC":19913, "CGCGG":24896,
"CGGAA":7547, "CGGAT":5725, "CGGAC":18619, "CGGAG":19656, "CGGTA":3600, "CGGTT":5417, "CGGTC":8541, "CGGTG":13881, "CGGCA":9527, "CGGCT":15488, "CGGCC":23384, "CGGCG":29890, "CGGGA":16696, "CGGGT":13060, "CGGGC":25377, "CGGGG":27474,
"GAAAA":21213, "GAAAT":16427, "GAAAC":14522, "GAAAG":18036, "GAATA":9437, "GAATT":30707, "GAATC":10642, "GAATG":12807, "GAACA":13337, "GAACT":18080, "GAACC":13968, "GAACG":11796, "GAAGA":18744, "GAAGT":13329, "GAAGC":16741, "GAAGG":21545,
"GATAA":8648, "GATAT":6541, "GATAC":5628, "GATAG":7024, "GATTA":9462, "GATTT":12340, "GATTC":9404, "GATTG":8783, "GATCA":10262, "GATCT":11166, "GATCC":10490, "GATCG":15868, "GATGA":10348, "GATGT":9470, "GATGC":9066, "GATGG":17904,
"GACAA":9035, "GACAT":8510, "GACAC":9372, "GACAG":16639, "GACTA":7629, "GACTT":10980, "GACTC":13562, "GACTG":19322, "GACCA":11776, "GACCT":12234, "GACCC":13634, "GACCG":7389, "GACGA":4049, "GACGT":4429, "GACGC":8514, "GACGG":10813,
"GAGAA":38764, "GAGAT":14403, "GAGAC":17892, "GAGAG":24996, "GAGTA":9034, "GAGTT":13035, "GAGTC":13224, "GAGTG":19067, "GAGCA":15126, "GAGCT":18533, "GAGCC":26275, "GAGCG":17009, "GAGGA":24972, "GAGGT":20654, "GAGGC":37347, "GAGGG":27249,
"GTAAA":10547, "GTAAT":10439, "GTAAC":6545, "GTAAG":8358, "GTATA":6618, "GTATT":9656, "GTATC":5184, "GTATG":6880, "GTACA":7157, "GTACT":6630, "GTACC":6177, "GTACG":3118, "GTAGA":15905, "GTAGT":8953, "GTAGC":10969, "GTAGG":10541,
"GTTAA":8162, "GTTAT":6898, "GTTAC":6113, "GTTAG":8394, "GTTTA":9251, "GTTTT":19947, "GTTTC":14331, "GTTTG":14109, "GTTCA":11048, "GTTCT":12625, "GTTCC":11989, "GTTCG":6306, "GTTGA":9417, "GTTGT":10683, "GTTGC":12423, "GTTGG":15550,
"GTCAA":6635, "GTCAT":8479, "GTCAC":9947, "GTCAG":13613, "GTCTA":5814, "GTCTT":12926, "GTCTC":17522, "GTCTG":14793, "GTCCA":8907, "GTCCT":12884, "GTCCC":16234, "GTCCG":9116, "GTCGA":3284, "GTCGT":4582, "GTCGC":10819, "GTCGG":20716,
"GTGAA":14021, "GTGAT":13228, "GTGAC":10845, "GTGAG":20612, "GTGTA":8683, "GTGTT":13723, "GTGTC":12433, "GTGTG":21577, "GTGCA":13853, "GTGCT":16268, "GTGCC":16625, "GTGCG":10905, "GTGGA":17835, "GTGGT":19107, "GTGGC":25365, "GTGGG":27669,
"GCAAA":11570, "GCAAT":8364, "GCAAC":9518, "GCAAG":12213, "GCATA":6349, "GCATT":9597, "GCATC":8379, "GCATG":12684, "GCACA":12544, "GCACT":13288, "GCACC":26352, "GCACG":8101, "GCAGA":18007, "GCAGT":17097, "GCAGC":27068, "GCAGG":29259,
"GCTAA":9160, "GCTAT":6535, "GCTAC":9240, "GCTAG":8824, "GCTTA":7170, "GCTTT":13830, "GCTTC":15505, "GCTTG":14619, "GCTCA":16188, "GCTCT":16997, "GCTCC":19541, "GCTCG":11753, "GCTGA":19487, "GCTGT":18698, "GCTGC":30840, "GCTGG":38524,
"GCCAA":12734, "GCCAT":14328, "GCCAC":18332, "GCCAG":24206, "GCCTA":8674, "GCCTT":14931, "GCCTC":27897, "GCCTG":29958, "GCCCA":19883, "GCCCT":18959, "GCCCC":24258, "GCCCG":21372, "GCCGA":12085, "GCCGT":7955, "GCCGC":28683, "GCCGG":27462,
"GCGAA":4968, "GCGAT":7026, "GCGAC":7099, "GCGAG":14569, "GCGTA":2608, "GCGTT":5511, "GCGTC":9016, "GCGTG":12525, "GCGCA":10132, "GCGCT":14317, "GCGCC":24016, "GCGCG":24114, "GCGGA":15490, "GCGGT":12733, "GCGGC":38964, "GCGGG":32408,
"GGAAA":19795, "GGAAT":13251, "GGAAC":11879, "GGAAG":24410, "GGATA":7471, "GGATT":13082, "GGATC":15826, "GGATG":13940, "GGACA":13007, "GGACT":23052, "GGACC":13457, "GGACG":9852, "GGAGA":26088, "GGAGT":20345, "GGAGC":29087, "GGAGG":43542,
"GGTAA":9220, "GGTAT":6562, "GGTAC":6115, "GGTAG":10896, "GGTTA":7510, "GGTTT":15964, "GGTTC":13173, "GGTTG":13890, "GGTCA":10816, "GGTCT":14542, "GGTCC":13653, "GGTCG":10738, "GGTGA":18866, "GGTGT":16395, "GGTGC":18558, "GGTGG":33543,
"GGCAA":11866, "GGCAT":11781, "GGCAC":25609, "GGCAG":30713, "GGCTA":8781, "GGCTT":14573, "GGCTC":23270, "GGCTG":41510, "GGCCA":21231, "GGCCT":21138, "GGCCC":26729, "GGCCG":26847, "GGCGA":13090, "GGCGT":11751, "GGCGC":27804, "GGCGG":44764,
"GGGAA":21489, "GGGAT":16725, "GGGAC":17734, "GGGAG":39822, "GGGTA":8665, "GGGTT":17071, "GGGTC":17680, "GGGTG":25501, "GGGCA":19855, "GGGCT":24711, "GGGCC":29960, "GGGCG":29327, "GGGGA":27124, "GGGGT":20831, "GGGGC":33446, "GGGGG":31304
},
"overrepresented_sequences": {
"AAAAAAAAAAAAAAAAAAAA":34,
"CTTGGCACCCGAGAATTCCA":128,
"GTCGGACTGTAGAACTCTGA":31,
"TCGGACTGTAGAACTCTGAA":54 }
},
"command": "fastp --overrepresentation_analysis --thread 1 --in1 /sfs/lustre/allocations/shefflab/processed/peppro_tutorial/pe/07-31-19/results_pipeline/tutorial/fastq/tutorial_R2.fastq --adapter_sequence GATCGTCGGACTGTAGAACTCTGAAC --length_required 26 --html /sfs/lustre/allocations/shefflab/processed/peppro_tutorial/pe/07-31-19/results_pipeline/tutorial/fastqc/tutorial_R2_rmAdapter.html --json /sfs/lustre/allocations/shefflab/processed/peppro_tutorial/pe/07-31-19/results_pipeline/tutorial/fastqc/tutorial_R2_rmAdapter.json --report_title tutorial --stdout "
}