FastQCFastQC Report
Thu 11 Jun 2020
H9_PRO-seq_10_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_10_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830874
Sequences flagged as poor quality0
Sequence length10-38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326631.1538132746282597No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN217250.7674308358478689No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA125870.44463300026776187No Hit
GGCACCCGAGAATTCCA121730.4300085415316966Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT97510.3444519254477592No Hit
GCACCCGAGAATTCCA75990.2684329998438645Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA65650.23190717778325703Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA56770.20053877353778377Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA50570.17863741021324156Illumina Small RNA Adapter 2 (100% over 14bp)
CGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT36500.1289354453783531No Hit
CCCGAGAATTCCA31750.11215617508938935Illumina Small RNA Adapter 2 (100% over 13bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)