FastQCFastQC Report
Thu 11 Jun 2020
H9_PRO-seq_20_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_20_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5665407
Sequences flagged as poor quality0
Sequence length10-38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA655441.1569159991506346No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN431770.762116472832402No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA249830.44097449662486743No Hit
GGCACCCGAGAATTCCA242650.4283010911660892Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT196700.3471948264264156No Hit
GCACCCGAGAATTCCA152270.2687715110317758Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA129900.22928626310519262Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA115320.2035511305719077Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA102210.18041069247099106Illumina Small RNA Adapter 2 (100% over 14bp)
CGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT72230.12749304683670565No Hit
CCCGAGAATTCCA63600.1122602489106255Illumina Small RNA Adapter 2 (100% over 13bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)