FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_3_R2_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_3_R2_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28703909
Sequences flagged as poor quality0
Sequence length10-30
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN6851502.3869571214150658No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC4748401.6542694585605047No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTT658090.22926842472918932No Hit
CGCGGGCCCAGAAGCGCAGCGACAGCCTC493400.1718929641255482No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN306230.10668581760066198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)