FastQCFastQC Report
Thu 11 Jun 2020
H9_PRO-seq_30_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_30_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8498087
Sequences flagged as poor quality0
Sequence length10-38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA981391.1548363767045453No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN644830.7587943027648458No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA373220.43918119454413684No Hit
GGCACCCGAGAATTCCA363500.427743326233304Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT293590.3454777528166045No Hit
GCACCCGAGAATTCCA229540.2701078489782465Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA197030.2318521803789488Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA174060.20482256771435736Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA152000.17886378428462782Illumina Small RNA Adapter 2 (100% over 14bp)
CGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT107590.12660496415252045No Hit
CCCGAGAATTCCA95720.11263711468239852Illumina Small RNA Adapter 2 (100% over 13bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)