FastQCFastQC Report
Thu 11 Jun 2020
H9_PRO-seq_30_R2_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_30_R2_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9256571
Sequences flagged as poor quality0
Sequence length10-30
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN2629772.84097642636782No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC2130172.301251727016408No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTT216230.23359622045787798No Hit
CGCGGGCCCAGAAGCGCAGCGACAGCCTC161610.17458948891549583No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN121120.1308475892422799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)