FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_70_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_70_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19833681
Sequences flagged as poor quality0
Sequence length10-38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2292361.1557915043606881No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN1498980.755774987003169No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA869970.4386326471621682No Hit
GGCACCCGAGAATTCCA849240.42818072953779984Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT686050.345901499575394No Hit
GCACCCGAGAATTCCA531640.26804908276986Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA461400.23263457751488492Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA408190.20580647636714539Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA355080.17902879450365264Illumina Small RNA Adapter 2 (100% over 14bp)
CGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT248780.12543309534926977No Hit
CCCGAGAATTCCA225060.11347364112592111Illumina Small RNA Adapter 2 (100% over 13bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)