FastQCFastQC Report
Mon 15 Jun 2020
H9_PRO-seq_80_R2_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_80_R2_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24685819
Sequences flagged as poor quality0
Sequence length10-30
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN7002142.8365030141394136No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC5685302.303063147307367No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTT574840.23286243814718077No Hit
CGCGGGCCCAGAAGCGCAGCGACAGCCTC431540.1748129158688233No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN321930.1304109051435563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)